Article
In planta protein sialylation through overexpression of the respective mammalian pathway.
The Journal of biological chemistry - 21 May 2010
Castilho Alexandra, Strasser Richard, Stadlmann Johannes, Grass Josephine, Jez Jakub, Gattinger Pia, Kunert Renate, Quendler Heribert, Pabst Martin, Leonard Renaud, Altmann Friedrich, Steinkellner Herta
Abstract excerpt
Many therapeutic proteins are glycosylated and require terminal sialylation to attain full biological activity. Current manufacturing methods based on mammalian cell culture allow only limited control of this important posttranslational modification, which may lead to the generation of products with low efficacy. Here we report in vivo protein sialylation in plants, which have been shown to be well suited for the...
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