Article

Functional knockout of FUT8 in Chinese hamster ovary cells using CRISPR/Cas9 to produce a defucosylated antibody

2015-07-21

Abstract excerpt

We report the adaptation of the new CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR‐associated protein 9) system to disrupt the gene encoding fucosyltransferase 8 (FUT8), an α1,6‐fucosyltransferase that directs fucose addition to derived antibody Fc region asparagine 297, in Chinese hamster ovary (CHO) cells. Compared to previously reported homologous recombination or zinc‐finger nucle...

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Literature Corpus work: 9f83505f-1952-5506-a90e-6563fdd45780
DOI: 10.1002/elsc.201400218

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