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Article

Amplicon sequencing of colorectal cancer: variant calling in frozen and formalin-fixed samples.

PloS one - 1 Jan 2015

Betge Johannes, Kerr Grainne, Miersch Thilo, Leible Svenja, Erdmann Gerrit, Galata Christian L, Zhan Tianzuo, Gaiser Timo, Post Stefan, Ebert Matthias P, Horisberger Karoline, Boutros Michael

Abstract excerpt

Next generation sequencing (NGS) is an emerging technology becoming relevant for genotyping of clinical samples. Here, we assessed the stability of amplicon sequencing from formalin-fixed paraffin-embedded (FFPE) and paired frozen samples from colorectal cancer metastases with different analysis pipelines. 212 amplicon regions in 48 cancer related genes were sequenced with Illumina MiSeq using DNA isolated from...

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Identifiers

PMID: 26010451
DOI: 10.1371/journal.pone.0127146
PMCID: PMC4444292

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APC
KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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