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A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders Using the CRISPR-Cas System.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Sakane Yuto, Suzuki Ken-Ich T, Yamamoto Takashi

Abstract excerpt

Xenopus tropicalis is a versatile model organism for studying basic biology such as developmental biology and cell biology, and for biomedical research on human diseases. Current genome editing techniques enable researchers to easily perform gene targeting in various animals. Among them, gene knockout using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) (CRISPR-Cas)...

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PMID: 28643260
DOI: 10.1007/978-1-4939-7128-2_16

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