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Article

A non-inheritable maternal Cas9-based multiple-gene editing system in mice.

Scientific reports - 28 Jan 2016

Sakurai Takayuki, Kamiyoshi Akiko, Kawate Hisaka, Mori Chie, Watanabe Satoshi, Tanaka Megumu, Uetake Ryuichi, Sato Masahiro, Shindo Takayuki

Abstract excerpt

The CRISPR/Cas9 system is capable of editing multiple genes through one-step zygote injection. The preexisting method is largely based on the co-injection of Cas9 DNA (or mRNA) and guide RNAs (gRNAs); however, it is unclear how many genes can be simultaneously edited by this method, and a reliable means to generate transgenic (Tg) animals with multiple gene editing has yet to be developed. Here, we employed...

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Identifiers

PMID: 26817415
DOI: 10.1038/srep20011
PMCID: PMC4730228

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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