Article
A non-inheritable maternal Cas9-based multiple-gene editing system in mice.
Scientific reports - 28 Jan 2016
Sakurai Takayuki, Kamiyoshi Akiko, Kawate Hisaka, Mori Chie, Watanabe Satoshi, Tanaka Megumu, Uetake Ryuichi, Sato Masahiro, Shindo Takayuki
Abstract excerpt
The CRISPR/Cas9 system is capable of editing multiple genes through one-step zygote injection. The preexisting method is largely based on the co-injection of Cas9 DNA (or mRNA) and guide RNAs (gRNAs); however, it is unclear how many genes can be simultaneously edited by this method, and a reliable means to generate transgenic (Tg) animals with multiple gene editing has yet to be developed. Here, we employed...
Topics
- Animals
- CRISPR-Associated Proteins
- CRISPR-Cas Systems
- Female
- Fibroblasts
- Galactosyltransferases
- Gene Editing
- Gene Expression
- Genetic Loci
- Genome
- Hepatocytes
- INDEL Mutation
- Male
- Mice