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Article

Procedures for Generating CRISPR Mutants with Novel Spacers Acquired from Viruses or Plasmids.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Dupuis Marie-Ève, Barrangou Rodolphe, Moineau Sylvain

Abstract excerpt

CRISPR-Cas systems provide immunity in bacteria and archaea against nucleic acids in the form of viral genomes and plasmids, and influence their coevolution. The first main step of CRISPR-Cas activity is the immune adaptation through spacer(s) acquisition into an active CRISPR locus. This step is also mandatory for the final stage of CRISPR-Cas activity, namely interference. This chapter describes general...

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PMID: 25981475
DOI: 10.1007/978-1-4939-2687-9_13

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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