Article
Strong bias in the bacterial CRISPR elements that confer immunity to phage.
Nature communications - 1 Jan 2013
Paez-Espino David, Morovic Wesley, Sun Christine L, Thomas Brian C, Ueda Ken-ichi, Stahl Buffy, Barrangou Rodolphe, Banfield Jillian F
Abstract excerpt
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems provide adaptive immunity against phage via spacer-encoded CRISPR RNAs that are complementary to invasive nucleic acids. Here, we challenge Streptococcus thermophilus with a bacteriophage, and used PCR-based metagenomics to monitor phage-derived spacers daily for 15 days in two experiments. Spacers that target the host chromosome are...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.