Article
Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells.
Proceedings of the National Academy of Sciences of the United States of America - 14 Apr 2015
Hayashi Yohei, Caboni Laura, Das Debanu, Yumoto Fumiaki, Clayton Thomas, Deller Marc C, Nguyen Phuong, Farr Carol L, Chiu Hsiu-Ju, Miller Mitchell D, Elsliger Marc-André, Deacon Ashley M, Godzik Adam, Lesley Scott A, Tomoda Kiichiro, Conklin Bruce R, Wilson Ian A, Yamanaka Shinya, Fletterick Robert J
Abstract excerpt
NANOG (from Irish mythology Tír na nÓg) transcription factor plays a central role in maintaining pluripotency, cooperating with OCT4 (also known as POU5F1 or OCT3/4), SOX2, and other pluripotency factors. Although the physiological roles of the NANOG protein have been extensively explored, biochemical and biophysical properties in relation to its structural analysis are poorly understood. Here we determined the...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Cell Proliferation
- Cells, Cultured
- Cellular Reprogramming
- Crystallography, X-Ray