Article
In vitro mispairing specificity of O2-ethylthymidine.
Biochemistry - 5 May 1992
Grevatt P C, Solomon J J, Bhanot O S
Abstract excerpt
The O2-position of thymine is a major site of base alkylation by N-nitroso-alkylating agents, and its biological relevance remains obscure. The potential significance of this DNA damage was ascertained by studying in vitro DNA replication properties of O2-ethylthymidine (O2-Et-dT) site-specifically incorporated into a 36-nucleotide template. DNA replication was initiated eight nucleotides away from the O2-Et-dT...
Topics
- Base Sequence
- DNA
- DNA Polymerase I
- Hydrogen Bonding
- Magnesium
- Manganese
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oligodeoxyribonucleotides
- Templates, Genetic
- Thymidine