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Article

Development of a molecularly evolved, highly sensitive CaMKII FRET sensor with improved expression pattern.

PloS one - 1 Jan 2015

Shibata Akihiro C E, Maebashi Hiroshi K, Nakahata Yoshihisa, Nabekura Junichi, Murakoshi Hideji

Abstract excerpt

Genetically encoded fluorescence resonance energy transfer (FRET) biosensors have been successfully used to visualize protein activity in living cells. The sensitivity and accuracy of FRET measurements directly depend on biosensor folding efficiency, expression pattern, sensitivity, and dynamic range. Here, to improve the folding efficiency of the Ca2+/calmodulin-dependent protein kinase II alpha (CaMKIIα) FRET...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25799407
DOI: 10.1371/journal.pone.0121109
PMCID: PMC4370617

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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