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Article

ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement.

Scientific reports - 28 Jul 2017

Murakoshi Hideji, Shibata Akihiro C E

Abstract excerpt

Fluorescence lifetime imaging microscopy (FLIM)-based Förster resonance energy transfer (FRET) measurement (FLIM-FRET) is one of the powerful methods for imaging of intracellular protein activities such as protein-protein interactions and conformational changes. Here, using saturation mutagenesis, we developed a dark yellow fluorescent protein named ShadowY that can serve as an acceptor for FLIM-FRET. ShadowY is...

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PMID: 28754922
DOI: 10.1038/s41598-017-07002-4
PMCID: PMC5533704

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EGF

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