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Article

Flow cytometric analysis of genetic FRET detectors containing variable substrate sequences.

Biotechnology progress - 1 Jan 2000

Hong Lim Kok, Hsu Cheng-Kuo, Park Sheldon

Abstract excerpt

A genetic Fluorescence Resonance Energy Transfer (FRET) detector undergoes a post-translational modification (PTM)-induced conformational change that results in increased FRET. To test if the PTM-dependent FRET change can be quantified by flow cytometry, we purified and immobilized a genetic detector on microbeads and used flow cytometry to measure its FRET efficiency before and after Erk-2-mediated...

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PMID: 20574990
DOI: 10.1002/btpr.468

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OGT

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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