Article
Fluorescent protein-based methods for on-plate screening of gene insertion.
PloS one - 10 Dec 2010
Wong Stanley S C, Truong Kevin
Abstract excerpt
BACKGROUND: Unlike the commonly used method of blue-white screening for gene insertion, a fluorescent protein-based screening method offers a gain-of-function screening process without using any co-factors and a gene fusion product with a fluorescent protein reporter that is further useful in cell imaging studies. However, complications related to protein-folding efficiencies of the gene insert in fusion with...
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