Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Enhanced specialized transduction using recombineering in Mycobacterium tuberculosis.

mBio - 27 May 2014

Tufariello JoAnn M, Malek Adel A, Vilchèze Catherine, Cole Laura E, Ratner Hannah K, González Pablo A, Jain Paras, Hatfull Graham F, Larsen Michelle H, Jacobs William R

Abstract excerpt

UNLABELLED: G: enetic engineering has contributed greatly to our understanding of Mycobacterium tuberculosis biology and has facilitated antimycobacterial and vaccine development. However, methods to generate M. tuberculosis deletion mutants remain labor-intensive and relatively inefficient. Here, methods are described that significantly enhance the efficiency (greater than 100-fold) of recovering deletion...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Genetic Engineering
Share to Alleles

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Expression Regulation, Bacterial

Share to Genetic Loci

Share to Mycobacterium tuberculosis

Share to Plasmids

Share to Recombination, Genetic

Share to Transduction, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 24865558
DOI: 10.1128/mBio.01179-14
PMCID: PMC4045075

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Enhanced specialized transduction using recombineering in Mycobacterium tuberculosis.PMID 24865558Read ↗

Mycobacterial recombineering.Semantic proximity Read ↗

Specialized transduction designed for precise high-throughput unmarked deletions in Mycobacterium tuberculosis.Semantic proximity Read ↗

Specialized transduction: an efficient method for generating marked and unmarked targeted gene disruptions in Mycobacterium tuberculosis, M. bovis BCG and M. smegmatis.Semantic proximity Read ↗

Mycobacterial recombineering.Semantic proximity Read ↗

Production of unmarked mutations in mycobacteria using site-specific recombination.Semantic proximity Read ↗

Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination.Semantic proximity Read ↗

Improved bacterial recombineering by parallelized protein discovery.Semantic proximity Read ↗

Oligo-Mediated Recombineering and its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis.Semantic proximity Read ↗

Improved bacterial recombineering by parallelized protein discoverySemantic proximity Read ↗

Efficient method for targeted gene disruption by homologous recombination in Mycobacterium avium subspecie paratuberculosis.Semantic proximity Read ↗

A VERSATILE LIBRARY OF TETRACYCLINE-INDUCIBLE AND REPRESSIBLE VECTORS FOR FINE-TUNED GENE EXPRESSIONSemantic proximity Read ↗

Efficient allelic exchange and transposon mutagenesis in Mycobacterium avium by specialized transduction.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.