Article
Rapid recombination screening to test gene essentiality demonstrates that pyrH is essential in Mycobacterium tuberculosis.
Tuberculosis (Edinburgh, Scotland) - 1 Sept 2007
Robertson Dina, Carroll Paul, Parish Tanya
Abstract excerpt
The availability of the complete genome of Mycobacterium tuberculosis affords the possibility of screening genes for essentiality under defined conditions. We tested a rapid recombination method for screening and confirmation of gene essentiality which would be more amenable to higher throughput applications. Non-replicating vectors carrying the internal portion of a gene were used as recombination substrates....
Topics
- Genes, Bacterial
- Genes, Essential
- Genotype
- Humans
- Mycobacterium tuberculosis
- Polymerase Chain Reaction
- Recombination, Genetic