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Continuous monitoring of enzymatic reactions on surfaces by real-time flow cytometry: sortase a catalyzed protein immobilization as a case study.

Bioconjugate chemistry - 20 Aug 2014

Heck Tobias, Pham Phu-Huy, Hammes Frederik, Thöny-Meyer Linda, Richter Michael

Abstract excerpt

Only a few techniques, such as quartz crystal microbalance and surface plasmon resonance spectroscopy, enable the analysis of dynamic processes on solid supports. Here we have developed a straightforward assay based on flow cytometry to continuously follow enzymatic reactions directly on microparticle surfaces. We applied this real-time flow cytometry (RT-FCM) approach to study the covalent immobilization of...

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PMID: 25075751
DOI: 10.1021/bc500230r

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