Article
Chemical genomic profiling via barcode sequencing to predict compound mode of action.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Piotrowski Jeff S, Simpkins Scott W, Li Sheena C, Deshpande Raamesh, McIlwain Sean J, Ong Irene M, Myers Chad L, Boone Charlie, Andersen Raymond J
Abstract excerpt
Chemical genomics is an unbiased, whole-cell approach to characterizing novel compounds to determine mode of action and cellular target. Our version of this technique is built upon barcoded deletion mutants of Saccharomyces cerevisiae and has been adapted to a high-throughput methodology using next-generation sequencing. Here we describe the steps to generate a chemical genomic profile from a compound of...
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