Article
Real-time resolution of point mutations that cause phenovariance in mice.
Proceedings of the National Academy of Sciences of the United States of America - 3 Feb 2015
Wang Tao, Zhan Xiaowei, Bu Chun-Hui, Lyon Stephen, Pratt David, Hildebrand Sara, Choi Jin Huk, Zhang Zhao, Zeng Ming, Wang Kuan-wen, Turer Emre, Chen Zhe, Zhang Duanwu, Yue Tao, Wang Ying, Shi Hexin, Wang Jianhui, Sun Lei, SoRelle Jeff, McAlpine William, Hutchins Noelle, Zhan Xiaoming, Fina Maggy, Gobert Rochelle, Quan Jiexia, Kreutzer McKensie, Arnett Stephanie, Hawkins Kimberly, Leach Ashley, Tate Christopher, Daniel Chad, Reyna Carlos, Prince Lauren, Davis Sheila, Purrington Joel, Bearden Rick, Weatherly Jennifer, White Danielle, Russell Jamie, Sun Qihua, Tang Miao, Li Xiaohong, Scott Lindsay, Moresco Eva Marie Y, McInerney Gerald M, Karlsson Hedestam Gunilla B, Xie Yang, Beutler Bruce
Abstract excerpt
With the wide availability of massively parallel sequencing technologies, genetic mapping has become the rate limiting step in mammalian forward genetics. Here we introduce a method for real-time identification of N-ethyl-N-nitrosourea-induced mutations that cause phenotypes in mice. All mutations are identified by whole exome G1 progenitor sequencing and their zygosity is established in G2/G3 mice before...
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