Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Improved bacterial recombineering by parallelized protein discovery.

Proceedings of the National Academy of Sciences of the United States of America - 16 Jun 2020

Wannier Timothy M, Nyerges Akos, Kuchwara Helene M, Czikkely Márton, Balogh Dávid, Filsinger Gabriel T, Borders Nathaniel C, Gregg Christopher J, Lajoie Marc J, Rios Xavier, Pál Csaba, Church George M

Abstract excerpt

Exploiting bacteriophage-derived homologous recombination processes has enabled precise, multiplex editing of microbial genomes and the construction of billions of customized genetic variants in a single day. The techniques that enable this, multiplex automated genome engineering (MAGE) and directed evolution with random genomic mutations (DIvERGE), are however, currently limited to a handful of microorganisms...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Engineering

Share to Genome, Bacterial

Share to Mutation

Share to Pseudomonas aeruginosa

Share to Recombination, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 32467157
DOI: 10.1073/pnas.2001588117
PMCID: PMC7306799

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Improved bacterial recombineering by parallelized protein discovery.PMID 32467157Read ↗

Improved bacterial recombineering by parallelized protein discoverySemantic proximity Read ↗

High-throughput functional variant screens via in vivo production of single-stranded DNA.Semantic proximity Read ↗

MOSAIC: A Highly Efficient, One-Step Recombineering Approach to Plasmid Editing and Diversification.Semantic proximity Read ↗

Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated CounterselectionSemantic proximity Read ↗

CRISPR/Cas9 recombineering-mediated deep mutational scanning of essential genes in Escherichia coli.Semantic proximity Read ↗

Strategies to identify and edit improvements in synthetic genome segments episomally.Semantic proximity Read ↗

Multiplex genome editing by natural transformation.Semantic proximity Read ↗

Efficient dual-negative selection for bacterial genome editing.Semantic proximity Read ↗

Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins.Semantic proximity Read ↗

Make-or-break prime editing for bacterial genome engineeringSemantic proximity Read ↗

Continuous Multiplexed Phage Genome Editing Using RecombitronsSemantic proximity Read ↗

Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.