Article
A Rapid and Cheap Methodology for CRISPR/Cas9 Zebrafish Mutant Screening.
Molecular biotechnology - 1 Jan 2016
D'Agostino Ylenia, Locascio Annamaria, Ristoratore Filomena, Sordino Paolo, Spagnuolo Antonietta, Borra Marco, D'Aniello Salvatore
Abstract excerpt
The introduction of new genome editing tools such as ZFNs, TALENs and, more recently, the CRISPR/Cas9 system, has greatly expanded the ability to knock-out genes in different animal models, including zebrafish. However, time and costs required for the screening of a huge number of animals, aimed to identify first founder fishes (F0), and then carriers (F1) are still a bottleneck. Currently, high-resolution...
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