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Article

In vitro amplification of scrapie and chronic wasting disease PrP(res) using baculovirus-expressed recombinant PrP as substrate.

Prion - 1 Jan 2014

Faburay Bonto, Tark Dongseob, Kanthasamy Anumantha G, Richt Juergen A

Abstract excerpt

Protein misfolding cyclic amplification (PMCA) is an in vitro simulation of prion replication, which relies on the use of normal brain homogenate derived from host species as substrate for the specific amplification of abnormal prion protein, PrP(Sc). Studies showed that recombinant cellular PrP, PrP(C), expressed in Escherichia coli lacks N-glycosylation and an glycophosphatidyl inositol anchor (GPI) and...

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Source

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Identifiers

PMID: 25495764
DOI: 10.4161/19336896.2014.983753
PMCID: PMC4601224

Genes

GPI
PRNP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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