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Article

A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing.

BMC genomics - 20 Nov 2014

Bell Charles C, Magor Graham W, Gillinder Kevin R, Perkins Andrew C

Abstract excerpt

BACKGROUND: CRISPR-Cas9 is a revolutionary genome editing technique that allows for efficient and directed alterations of the eukaryotic genome. This relatively new technology has already been used in a large number of 'loss of function' experiments in cultured cells. Despite its simplicity and efficiency, screening for mutated clones remains time-consuming, laborious and/or expensive. RESULTS: Here we report a...

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Identifiers

PMID: 25409780
DOI: 10.1186/1471-2164-15-1002
PMCID: PMC4246457

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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