Article
Single cell gel electrophoresis (SCGE) and Pig-a mutation assay in vivo-tools for genotoxicity testing from a regulatory perspective: a study of benzo[a]pyrene in Ogg1(-/-) mice.
Mutation research. Genetic toxicology and environmental mutagenesis - 15 Sept 2014
Graupner Anne, Instanes Christine, Dertinger Stephen D, Andersen Jill Mari, Lindeman Birgitte, Rongved Tonje Danielsen, Brunborg Gunnar, Olsen Ann-Karin
Abstract excerpt
The OECD has developed test guidelines (TG) to identify agents with genotoxic effects. The in vivo alkaline single cell gel electrophoresis (SCGE) assay is currently being prepared to become such a TG. The performance of a combined SCGE/Pig-a gene mutation study was evaluated with the prototypica...
Topics
- Animals
- Benzo(a)pyrene
- DNA Damage
- DNA Glycosylases
- Electrophoresis
- Erythrocytes, Abnormal
- Membrane Proteins
- Mice
- Mice, Knockout
- Mutagens
- Mutation
- Oxidative Stress
- Reactive Oxygen Species