Article
Confirmation of the cellular targets of benomyl and rapamycin using next-generation sequencing of resistant mutants in S. cerevisiae.
Molecular bioSystems - 1 Dec 2014
Wride Dustin A, Pourmand Nader, Bray Walter M, Kosarchuk Jacob J, Nisam Sean C, Quan Tiffani K, Berkeley Ray F, Katzman Sol, Hartzog Grant A, Dobkin Carlos E, Scott Lokey R
Abstract excerpt
Investigating the mechanisms of action (MOAs) of bioactive compounds and the deconvolution of their cellular targets is an important and challenging undertaking. Drug resistance in model organisms such as S. cerevisiae has long been a means for discovering drug targets and MOAs. Strains are selected for resistance to a drug of interest, and the resistance mutations can often be mapped to the drug's molecular...
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