Article
High-Resolution Phenotypic Landscape of the RNA Polymerase II Trigger Loop.
PLoS genetics - 1 Nov 2016
Qiu Chenxi, Erinne Olivia C, Dave Jui M, Cui Ping, Jin Huiyan, Muthukrishnan Nandhini, Tang Leung K, Babu Sabareesh Ganesh, Lam Kenny C, Vandeventer Paul J, Strohner Ralf, Van den Brulle Jan, Sze Sing-Hoi, Kaplan Craig D
Abstract excerpt
The active sites of multisubunit RNA polymerases have a "trigger loop" (TL) that multitasks in substrate selection, catalysis, and translocation. To dissect the Saccharomyces cerevisiae RNA polymerase II TL at individual-residue resolution, we quantitatively phenotyped nearly all TL single variants en masse. Three mutant classes, revealed by phenotypes linked to transcription defects or various stresses, have...
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