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Article

Measuring genetic interactions in human cells by RNAi and imaging.

Nature protocols - 1 Oct 2014

Laufer Christina, Fischer Bernd, Huber Wolfgang, Boutros Michael

Abstract excerpt

Observation of how genetic interactions modulate phenotypes is a powerful method for dissecting their underlying molecular and functional networks. Whereas in model organisms genetic interaction studies are well established, systematic analysis of genetic interactions in human cells has remained challenging. Here we provide a detailed protocol for large-scale mapping of genetic interactions in human cells using a...

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PMID: 25211512
DOI: 10.1038/nprot.2014.160

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