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Article

Rapid sequencing of the Mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility.

Journal of clinical microbiology - 1 Nov 2014

Streicher Elizabeth M, Maharaj Kashmeel, York Talita, Van Heerden Carel, Barnard Marinus, Diacon Andreas, Mendel Carl M, Bosman Marlein E, Hepple Juli A, Pym Alexander S, Warren Robin M, van Helden Paul D

Abstract excerpt

We developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous mutations and nonsynonymous mutations previously associated with susceptibility to...

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Identifiers

PMID: 25165081
DOI: 10.1128/JCM.02438-14
PMCID: PMC4313251

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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