Article
Ultrafast water dynamics at the interface of the polymerase-DNA binding complex.
Biochemistry - 26 Aug 2014
Yang Yi, Qin Yangzhong, Ding Qing, Bakhtina Marina, Wang Lijuan, Tsai Ming-Daw, Zhong Dongping
Abstract excerpt
DNA polymerases slide on DNA during replication, and the interface must be mobile for various conformational changes. The role of lubricant interfacial water is not understood. In this report, we systematically characterize the water dynamics at the interface and in the active site of a tight binding polymerase (pol β) in its binary complex and ternary state using tryptophan as a local optical probe. Using...
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