Article
Assembly principles of a unique cage formed by hexameric and decameric E. coli proteins.
eLife - 5 Aug 2014
Malet Hélène, Liu Kaiyin, El Bakkouri Majida, Chan Sze Wah Samuel, Effantin Gregory, Bacia Maria, Houry Walid A, Gutsche Irina
Abstract excerpt
A 3.3 MDa macromolecular cage between two Escherichia coli proteins with seemingly incompatible symmetries-the hexameric AAA+ ATPase RavA and the decameric inducible lysine decarboxylase LdcI-is reconstructed by cryo-electron microscopy to 11 Å resolution. Combined with a 7.5 Å resolution reconstruction of the minimal complex between LdcI and the LdcI-binding domain of RavA, and the previously solved crystal...
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