Article
Clonal evolution in breast cancer revealed by single nucleus genome sequencing.
Nature - 14 Aug 2014
Wang Yong, Waters Jill, Leung Marco L, Unruh Anna, Roh Whijae, Shi Xiuqing, Chen Ken, Scheet Paul, Vattathil Selina, Liang Han, Multani Asha, Zhang Hong, Zhao Rui, Michor Franziska, Meric-Bernstam Funda, Navin Nicholas E
Abstract excerpt
Sequencing studies of breast tumour cohorts have identified many prevalent mutations, but provide limited insight into the genomic diversity within tumours. Here we developed a whole-genome and exome single cell sequencing approach called nuc-seq that uses G2/M nuclei to achieve 91% mean coverage breadth. We applied this method to sequence single normal and tumour nuclei from an oestrogen-receptor-positive...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.