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Article

False positives in multiplex PCR-based next-generation sequencing have unique signatures.

The Journal of molecular diagnostics : JMD - 1 Sept 2014

McCall Chad M, Mosier Stacy, Thiess Michele, Debeljak Marija, Pallavajjala Aparna, Beierl Katie, Deak Kristen L, Datto Michael B, Gocke Christopher D, Lin Ming-Tseh, Eshleman James R

Abstract excerpt

Next-generation sequencing shows great promise by allowing rapid mutational analysis of multiple genes in human cancers. Recently, we implemented the multiplex PCR-based Ion AmpliSeq Cancer Hotspot Panel (>200 amplicons in 50 genes) to evaluate EGFR, KRAS, and BRAF in lung and colorectal adenocarcinomas. In 10% of samples, automated analysis identified a novel G873R substitution mutation in EGFR. By examining...

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Identifiers

PMID: 25017478
DOI: 10.1016/j.jmoldx.2014.06.001
PMCID: PMC4188281

Genes

EGFR
BRAF
KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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