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Article

Potential pitfalls in multiplex PCR-based next-generation sequencing: a case-based report.

Journal of clinical pathology - 1 Jan 2023

Tung Jack K, Devereaux Kelly A, Erdmann Archana Lal, Schrijver Iris, Zehnder James, Suarez Carlos J

Abstract excerpt

Amplicon-based next-generation sequencing (NGS) assays employ highly sensitive, rapid, and cost-effective methods to detect clinically actionable mutations for the diagnosis, prognosis, and treatment of patients with cancer. However, recognition of certain limitations inherent to amplicon-based NGS assays is crucial for the correct interpretation and reporting of variants in the clinical setting. In this report,...

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PMID: 35145018
DOI: 10.1136/jclinpath-2021-208105

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