Article
Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9.
Experimental animals - 1 Jan 2015
Honda Arata, Hirose Michiko, Sankai Tadashi, Yasmin Lubna, Yuzawa Kazuaki, Honsho Kimiko, Izu Haruna, Iguchi Atsushi, Ikawa Masahito, Ogura Atsuo
Abstract excerpt
Targeted genome editing of nonrodent mammalian species has provided the potential for highly accurate interventions into gene function in humans and the generation of useful animal models of human diseases. Here we show successful clustered regularly interspaced short palindromic repeat (CRISPR)...
Topics
- Alleles
- Animals
- CRISPR-Associated Proteins
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Disease Models, Animal
- Gene Targeting
- Monophenol Monooxygenase
- Plasmids
- Rabbits
- Zygote