Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state.

Nucleic acids research - 1 Jul 2014

Mahyous Saeyd Salah Adam, Ewert-Krzemieniewska Katarzyna, Liu Boyin, Caspari Thomas

Abstract excerpt

Although it is well established that Cdc2 kinase phosphorylates the DNA damage checkpoint protein Crb2(53BP1) in mitosis, the full impact of this modification is still unclear. The Tudor-BRCT domain protein Crb2 binds to modified histones at DNA lesions to mediate the activation of Chk1 by Rad3ATR kinase. We demonstrate here that fission yeast cells harbouring a hyperactive Cdc2CDK1 mutation (cdc2.1w) are...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antigens, Nuclear
Share to Camptothecin

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CDC2 Protein Kinase

Share to Checkpoint Kinase 1

Share to G1 Phase

Share to G2 Phase Cell Cycle Checkpoints

Share to Ku Autoantigen

Share to Topoisomerase I Inhibitors

Source

Open in PubMed Open full text

Identifiers

PMID: 24861625
DOI: 10.1093/nar/gku452
PMCID: PMC4081076

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state.PMID 24861625Read ↗

Cooperative control of Crb2 by ATM family and Cdc2 kinases is essential for the DNA damage checkpoint in fission yeast.Semantic proximity Read ↗

The fission yeast Crb2/Chk1 pathway coordinates the DNA damage and spindle checkpoint in response to replication stress induced by topoisomerase I inhibitor.Semantic proximity Read ↗

Regulation of checkpoint kinases through dynamic interaction with Crb2.Semantic proximity Read ↗

BRCT domain interactions with phospho-histone H2A target Crb2 to chromatin at double-strand breaks and maintain the DNA damage checkpoint.Semantic proximity Read ↗

Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint.Semantic proximity Read ↗

Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1.Semantic proximity Read ↗

Fission yeast chk1 mutants show distinct responses to different types of DNA damaging treatments.Semantic proximity Read ↗

Requirement for the phospho-H2AX binding module of Crb2 in double-strand break targeting and checkpoint activation.Semantic proximity Read ↗

Structural and functional analysis of the Crb2-BRCT2 domain reveals distinct roles in checkpoint signaling and DNA damage repair.Semantic proximity Read ↗

The S/M checkpoint at 37 degrees C and the recovery of viability of the mutant poldeltats3 require the crb2+/rhp9+ gene in fission yeast.Semantic proximity Read ↗

Rad4TopBP1, a scaffold protein, plays separate roles in DNA damage and replication checkpoints and DNA replication.Semantic proximity Read ↗

Histone H2A phosphorylation controls Crb2 recruitment at DNA breaks, maintains checkpoint arrest, and influences DNA repair in fission yeast.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.