Article
Glyco-variant library of the versatile enzyme horseradish peroxidase.
Glycobiology - 1 Sept 2014
Capone Simona, Pletzenauer Robert, Maresch Daniel, Metzger Karl, Altmann Friedrich, Herwig Christoph, Spadiut Oliver
Abstract excerpt
When the glycosylated plant enzyme horseradish peroxidase (HRP) is conjugated to specific antibodies, it presents a powerful tool for medical applications. The isolation and purification of this enzyme from plant is difficult and only gives low yields. However, HRP recombinantly produced in the yeast Pichia pastoris experiences hyperglycosylation, which impedes the use of this enzyme in medicine. Enzymatic and...
Topics
- Amino Acid Motifs
- Biotechnology
- Glycosylation
- Horseradish Peroxidase
- Mutation
- Pichia
- Plant Proteins
- Protein Engineering
- Protein Processing, Post-Translational
- Recombinant Proteins