Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris.

doi:10.1038/srep03279

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Article

Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris.

Scientific reports - 20 Nov 2013

Krainer Florian W, Gmeiner Christoph, Neutsch Lukas, Windwarder Markus, Pletzenauer Robert, Herwig Christoph, Altmann Friedrich, Glieder Anton, Spadiut Oliver

Abstract excerpt

The yeast Pichia pastoris is a common host for the recombinant production of biopharmaceuticals, capable of performing posttranslational modifications like glycosylation of secreted proteins. However, the activity of the OCH1 encoded α-1,6-mannosyltransferase triggers hypermannosylation of secreted proteins at great heterogeneity, considerably hampering downstream processing and reproducibility. Horseradish...

Topics

Batch Cell Culture Techniques
Bioreactors
Cell Division
Chromatography, Liquid
Enzyme Activation
Fungal Proteins
Gene Knockout Techniques
Gene Order
Gene Targeting
Glycoproteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mannose-Binding Lectins

Mannosyltransferases

Mass Spectrometry

Phenotype

Pichia

Polysaccharides

Recombinant Proteins

Stress, Physiological

Source

Open in PubMed Open full text

Identifiers

PMID: 24252857
DOI: 10.1038/srep03279
PMCID: PMC3834888

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-24252857-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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