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Article

Probing active-site residues of pyranose 2-oxidase from Trametes multicolor by semi-rational protein design.

Biotechnology journal - 1 Apr 2009

Salaheddin Clara, Spadiut Oliver, Ludwig Roland, Tan Tien-Chye, Divne Christina, Haltrich Dietmar, Peterbauer Clemens

Abstract excerpt

D-Tagatose is a sweetener with low caloric and non-glycemic characteristics. It can be produced by an enzymatic oxidation of D-galactose specifically at C2 followed by chemical hydrogenation. Pyranose 2-oxidase (P2Ox) from Trametes multicolor catalyzes the oxidation of many aldopyranoses to their corresponding 2-keto derivatives. Since D-galactose is not the preferred substrate of P2Ox, semi-rational design was...

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PMID: 19370721
DOI: 10.1002/biot.200800265

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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