Article
Functional expression of a blood tolerant laccase in Pichia pastoris.
BMC biotechnology - 30 Apr 2013
Mate Diana M, Gonzalez-Perez David, Kittl Roman, Ludwig Roland, Alcalde Miguel
Abstract excerpt
BACKGROUND: Basidiomycete high-redox potential laccases (HRPLs) working in human physiological fluids (pH 7.4, 150 mM NaCl) arise great interest in the engineering of 3D-nanobiodevices for biomedical uses. In two previous reports, we described the directed evolution of a HRPL from basidiomycete PM1 strain CECT 2971: i) to be expressed in an active, soluble and stable form in Saccharomyces cerevisiae, and ii) to...
Topics
- Blood Physiological Phenomena
- Cloning, Molecular
- Directed Molecular Evolution
- Enzyme Stability
- Glycosylation
- Humans
- Kinetics
- Laccase
- Models, Molecular
- Mutation
- Pichia