Article
Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pastoris.
International journal of molecular sciences - 24 Sept 2015
Capone Simona, Ćorajević Lejla, Bonifert Günther, Murth Patrick, Maresch Daniel, Altmann Friedrich, Herwig Christoph, Spadiut Oliver
Abstract excerpt
Horseradish peroxidase (HRP), conjugated to antibodies and lectins, is widely used in medical diagnostics. Since recombinant production of the enzyme is difficult, HRP isolated from plant is used for these applications. Production in the yeast Pichia pastoris (P. pastoris), the most promising recombinant production platform to date, causes hyperglycosylation of HRP, which in turn complicates conjugation to...
Topics
- Bioreactors
- Cell Engineering
- Glycosylation
- Horseradish Peroxidase
- Mutation
- Pichia
- Protein Engineering
- Recombinant Proteins