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Chemoenzymatic synthesis of unusual antibody glycoforms using E. coli expressed human and bovine β-1,4-galactosyltransferase and their mutants.

Carbohydrate research - 1 Sept 2026

Natasha Owitipana D, Harris Darnell, Yun Helena, Zong Guanghui, Wang Lai-Xi

Abstract excerpt

Antibody Fc glycosylation critically influences effector functions, motivating the development of chemoenzymatic strategies to generate homogeneous glycoforms. However, limited availability and high cost of mammalian glycosyltransferases remain to be significant constraints. Here, we report the high-yield expression of human and bovine β-1,4-galactosyltransferases (B4GalT1) and their single-point mutants (Y285L...

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Identifiers

PMID: 42378775
DOI: 10.1016/j.carres.2026.110019
PMCID: PMC13439607

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