Article
[The detection of Leiden mutation using technique of enzymatic extension of allele-specific primer with double bioluminescent detection (PED-Biolum)].
Klinicheskaia laboratornaia diagnostika - 1 Dec 2013
Krasitskaia V V, Subbotina T N, Ol'khovskiĭ I A, Frank L A
Abstract excerpt
The article deals with comparing technique of detection of Leiden mutation on the basis of PEXT-reaction with subsequent bioluminescent microanalysis of products with technique based on RT-PCR. The sampling for testing comprised 83 specimen of genome DNA including 35 specimens with known Leiden heterozygote mutation. The commercial kit "SNP-express-PB" (Litex) was used as a comparison test. It is demonstrated...
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