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Rapid genotyping of factor V Leiden mutation using single-tube bidirectional allele-specific amplification and automated ultrathin-layer agarose gel electrophoresis.

Electrophoresis - 1 Mar 2000

Sasvari-Szekely M, Gerstner A, Ronai Z, Staub M, Guttman A

Abstract excerpt

We report a novel, high-throughput genotyping method by single nucleotide polymorphism (SNP) analysis using bidirectional allele-specific amplification with polymerase chain reaction (PCR) in a single-step/single-tube format. Blood coagulation factor V G1691A (also referred to as Leiden) mutation was chosen as a model system for SNP detection, as this is one of the most common inherited risk factors of...

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PMID: 10733227
DOI: 10.1002/(SICI)1522-2683(20000301)21:4<816::AID-ELPS816>3.0.CO;2-Y

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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