Article
[New DNA diagnostic system for determination and identification of homo- and heterozygote point mutations].
Bioorganicheskaia khimiia - 1 Mar 1998
Patrushev L I, Zykova E S, Kaiushin A L, Korosteleva M D, Miroshnikov A I
Abstract excerpt
A straightforward and effective PCR-based assay system is devised that allows one to reveal and identify homozygous and heterozygous point mutations. The system uses two sets of allele-specific primers. In one set, the 3'-nucleotide matches the allele under study so that the primer functions effe...
Topics
- Alleles
- DNA
- DNA Primers
- Electrophoresis, Agar Gel
- Factor V
- Genetic Markers
- Heterozygote
- Homozygote
- Humans
- Oligonucleotides
- Point Mutation
- Polymerase Chain Reaction
- Thrombophilia