Article
[New DNA diagnostic system for determination and identification of homo- and heterozygote point mutations].
Bioorganicheskaia khimiia - 1 Mar 1998
Patrushev L I, Zykova E S, Kaiushin A L, Korosteleva M D, Miroshnikov A I
Abstract excerpt
A straightforward and effective PCR-based assay system is devised that allows one to reveal and identify homozygous and heterozygous point mutations. The system uses two sets of allele-specific primers. In one set, the 3'-nucleotide matches the allele under study so that the primer functions effectively only if the DNA contains the corresponding allele. To increase primer specificity, template-noncomplementary...
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