Article
New DNA diagnostic system for detection of factor V Leiden.
Thrombosis research - 15 Dec 1998
Patrushev L I, Zykova E S, Kayushin A L, Korosteleva M D, Miroshnikov A I, Bokarew I N, Leont'ev S G, Koshkin V M, Severin E S
Abstract excerpt
Use was made of allele-specific PCR to develop a highly effective DNA diagnostic system for detection of the factor V Leiden mutation in exon 10 of human factor V gene. The allele-specific primers contain a 3'-OH end nucleotide, which matches a mutant or wild-type nucleotide of the template DNA (...
Topics
- Alleles
- Biological Assay
- DNA
- Factor V
- Humans
- Oligonucleotide Probes
- Point Mutation