Article
New DNA diagnostic system for detection of factor V Leiden.
Thrombosis research - 15 Dec 1998
Patrushev L I, Zykova E S, Kayushin A L, Korosteleva M D, Miroshnikov A I, Bokarew I N, Leont'ev S G, Koshkin V M, Severin E S
Abstract excerpt
Use was made of allele-specific PCR to develop a highly effective DNA diagnostic system for detection of the factor V Leiden mutation in exon 10 of human factor V gene. The allele-specific primers contain a 3'-OH end nucleotide, which matches a mutant or wild-type nucleotide of the template DNA (A-allele and G-allele, respectively) and also one mismatched nucleotide near the 3'-end. The universal primers have an...
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