Article
Context and number of noncanonical repeat variable diresidues impede the design of TALE proteins with improved DNA targeting.
Protein science : a publication of the Protein Society - 1 Feb 2020
Anderson James T, Rogers Julia M, Barrera Luis A, Bulyk Martha L
Abstract excerpt
Transcription activator-like effector (TALE) proteins have been used extensively for targeted binding of fusion proteins to loci of interest in (epi)genome engineering. Such approaches typically utilize four canonical TALE repeat variable diresidue (RVD) types, corresponding to the identities of two key amino acids, to target each nucleotide. Alternate RVDs with improved specificity are desired. Here, we focused...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.