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Article

CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics.

The Analyst - 7 Jul 2014

Beneyton Thomas, Coldren Faith, Baret Jean-Christophe, Griffiths Andrew D, Taly Valérie

Abstract excerpt

We present a high-throughput droplet-based microfluidic analysis/screening platform for directed evolution of CotA laccase: droplet-based microfluidic modules were combined to develop an efficient system that allows cell detection and sorting based on the enzymatic activity. This platform was run on two different operating modes: the "analysis" mode allowing the analysis of the enzymatic activity in droplets at...

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Bacillus subtilis
Directed Molecular Evolution
Equipment Design
Flow Cytometry
High-Throughput Screening Assays

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Microfluidic Analytical Techniques

Recombinant Proteins

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PMID: 24733162
DOI: 10.1039/c4an00228h

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