Recovery of phenotypically sorted cells using droplet-digital microfluidics.

Abstract excerpt

Droplet microfluidics has become a ubiquitous and powerful tool for high-throughput phenotypic screening at the single-cell level. Large numbers of cells can be sorted for a variety of functions, including the secretion of antibodies with tailored properties. The recovery of cells from sorted droplets is still very poor compared to droplet sorting, usually being limited to around 50% of all sorted hits. Here, we...

Topics

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• Cell Separation
• Microfluidic Analytical Techniques
• Phenotype
• Single-Cell Analysis