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Article

Use of photoswitchable fluorescent proteins for droplet-based microfluidic screening.

Journal of microbiological methods - 1 Apr 2018

Dagkesamanskaya Adilya, Langer Krzysztof, Tauzin Alexandra S, Rouzeau Catherine, Lestrade Delphine, Potocki-Veronese Gabrielle, Boitard Laurent, Bibette Jérôme, Baudry Jean, Pompon Denis, Anton-Leberre Véronique

Abstract excerpt

Application of droplet-based microfluidics for the screening of microbial libraries is one of the important ongoing developments in functional genomics/metagenomics. In this article, we propose a new method that can be employed for high-throughput profiling of cell growth. It consists of light-driven labelling droplets that contain growing cells directly in a microfluidics observation chamber, followed by...

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Gene Library
Green Fluorescent Proteins
Luminescent Proteins
Metagenomics
Microfluidic Analytical Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Red Fluorescent Protein

Staining and Labeling

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PMID: 29518436
DOI: 10.1016/j.mimet.2018.03.001

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