Article
Inclusion body formation and protein stability in sequence variants of interleukin-1 beta.
The Journal of biological chemistry - 25 Aug 1993
Chrunyk B A, Evans J, Lillquist J, Young P, Wetzel R
Abstract excerpt
Inclusion body formation during recombinant protein expression in bacteria is of both fundamental interest and practical importance. To elucidate molecular mechanisms of this process, we are examining the in vitro folding and stability properties of a series of human interleukin-1 beta (IL-1 beta...
Topics
- Amino Acid Sequence
- Calorimetry
- Drug Stability
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genetic Variation
- Humans
- Inclusion Bodies
- Interleukin-1
- Models, Structural
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Plasmids
- Protein Structure, Secondary
- Recombinant Proteins
- Spectrometry, Fluorescence
- Tryptophan