Article
A reference-guided TILLING by amplicon-sequencing platform supports forward and reverse genetics in barley.
Plant communications - 11 Jul 2022
Jiang Congcong, Lei Miaomiao, Guo Yu, Gao Guangqi, Shi Lijie, Jin Yanlong, Cai Yu, Himmelbach Axel, Zhou Shenghui, He Qiang, Yao Xuefeng, Kan Jinhong, Haberer Georg, Duan Fengying, Li Lihui, Liu Jun, Zhang Jing, Spannagl Manuel, Liu Chunming, Stein Nils, Feng Zongyun, Mascher Martin, Yang Ping
Abstract excerpt
Barley is a diploid species with a genome smaller than those of other members of the Triticeae tribe, making it an attractive model for genetic studies in Triticeae crops. The recent development of barley genomics has created a need for a high-throughput platform to identify genetically uniform mutants for gene function investigations. In this study, we report an ethyl methanesulfonate (EMS)-mutagenized...
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